Hotspot

trnF-GAA-ndhJ

LSC · 49501-50137 · 637 bp

Back to Solanum

Pi 0.028

MarkerSeek score 62.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 637
variable_sites: 204
pi: 0.028
unique_haplotype_count: 98
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	AATAGCCGGGATAGCTCAGT	AAAGATGCAGGGTCGTTTGT	50.0	45.0	58.0	58.0	1156-1259 bp	0.994924	91.5
trnF-GAA-ndhJ_p2	ATAGCCGGGATAGCTCAGTT	AAAGATGCAGGGTCGTTTGT	50.0	45.0	58.0	58.0	1155-1258 bp	0.994924	91.5
trnF-GAA-ndhJ_p3	TTATCCGACCCCCTTTCCTT	AAAGATGCAGGGTCGTTTGT	50.0	45.0	58.0	58.0	1519-2014 bp	0.964467	90.2
trnF-GAA-ndhJ_p4	CGACCCGAATCCGTAGTTTT	AAAGATGCAGGGTCGTTTGT	50.0	45.0	58.0	58.0	1816-2297 bp	0.84264	85.6
trnF-GAA-ndhJ_p5	TGTTAGCGGTTCCAATCGAG	AAAGATGCAGGGTCGTTTGT	50.0	45.0	58.0	58.0	1220-1327 bp	0.664975	78.4