Hotspot

rpoB-trnC-GCA

LSC · 27225-28538 · 1314 bp

Back to Solanum

Pi 0.0172

MarkerSeek score 68.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1314
variable_sites: 351
pi: 0.0172
unique_haplotype_count: 137
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTCATTTCCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	57.9	50.0	58.0	58.1	1166-1430 bp	0.994924	83.0
rpoB-trnC-GCA_p2	TCATTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.2	58.1	1165-1429 bp	1.0	82.1
rpoB-trnC-GCA_p3	ATTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1171-1435 bp	0.979695	81.9
rpoB-trnC-GCA_p4	CTCATTTCCATCCCCGAGC	AGTCCTCTGCCTTACCACTC	57.9	55.0	58.0	58.4	1135-1399 bp	0.994924	80.2
rpoB-trnC-GCA_p5	CATTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	52.6	50.0	57.6	58.1	1164-1428 bp	1.0	79.9