Hotspot

rps16-trnQ-UUG

LSC · 6197-7215 · 1019 bp

Back to Solanum

Pi 0.0378
MarkerSeek score 82.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1019
variable_sites
464
pi
0.0378
unique_haplotype_count
163
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 697-1639 bp 1.0 90.7
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 680-1622 bp 1.0 90.3
rps16-trnQ-UUG_p3 GTCGAGCTAAGAGCACCTTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 751-1693 bp 0.989848 90.3
rps16-trnQ-UUG_p4 CAACGGATCGTGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 698-1640 bp 1.0 89.7
rps16-trnQ-UUG_p5 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 694-1636 bp 1.0 88.4