Hotspot

ycf2

IRb · 91528-98451 · 6924 bp

Back to Sobralia

Pi 0.00217

MarkerSeek score 54.00

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6924
variable_sites: 15
pi: 0.00217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TCGAATCCTACTGAGAGGTCC	CCTTTGTTCCGGTCATGGAA	52.4	50.0	58.1	58.1	284 bp	1.0	73.5
ycf2_p2	TCTGGGTTCGAATCCTACTGA	CCTTTGTTCCGGTCATGGAA	47.6	50.0	57.9	58.1	291 bp	1.0	73.2
ycf2_p3	TGGGTTCGAATCCTACTGAGA	CCTTTGTTCCGGTCATGGAA	47.6	50.0	57.9	58.1	289 bp	1.0	73.2
ycf2_p4	TGGTCAGAACTTCATCTGGG	CCTTTGTTCCGGTCATGGAA	50.0	50.0	56.8	58.1	305 bp	1.0	72.6
ycf2_p5	GGTCAGAACTTCATCTGGGT	CCTTTGTTCCGGTCATGGAA	50.0	50.0	56.8	58.1	304 bp	1.0	72.6