Hotspot

ycf1

SSC · 130056-135605 · 5550 bp

Back to Sobralia

Pi 0.00975

MarkerSeek score 47.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5550
variable_sites: 54
pi: 0.00975
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACTAGGTCCTTTTGAATAAGCA	TTTCCTTTTTCAACTAAGATGG	36.4	31.8	55.1	52.6	168 bp	1.0	42.4
ycf1_p2	AACTAGGTCCTTTTGAATAAGCA	TTTCCTTTTTCAACTAAGATGG	34.8	31.8	55.8	52.6	169 bp	1.0	42.4
ycf1_p3	ACTAGGTCCTTTTGAATAAGCA	ATTTCCTTTTTCAACTAAGATGG	36.4	30.4	55.1	53.2	169 bp	1.0	42.4
ycf1_p4	ACTAGGTCCTTTTGAATAAGCAT	TTTCCTTTTTCAACTAAGATGG	34.8	31.8	55.6	52.6	168 bp	1.0	42.4
ycf1_p5	AACTAGGTCCTTTTGAATAAGCA	ATTTCCTTTTTCAACTAAGATGG	34.8	30.4	55.8	53.2	170 bp	1.0	42.4