Hotspot

rps8

LSC · 84594-84989 · 396 bp

Back to Sobralia

Pi 0.00758

MarkerSeek score 49.00

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 396
variable_sites: 3
pi: 0.00758
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	TCGAGAGAAAATTAGGGTACCC	TCAAAATGAGGAGGCACCAA	45.5	45.0	57.0	57.3	461 bp	1.0	51.0
rps8_p2	GGAACCCCCTTTAAGTATCAACT	TCAAAATGAGGAGGCACCAA	43.5	45.0	57.8	57.3	539 bp	1.0	49.5
rps8_p3	TCGAGAGAAAATTAGGGTACCC	ATCAAAATGAGGAGGCACCA	45.5	45.0	57.0	57.1	462 bp	1.0	49.2
rps8_p4	GGAACCCCCTTTAAGTATCAACT	ATCAAAATGAGGAGGCACCA	43.5	45.0	57.8	57.1	540 bp	1.0	47.7
rps8_p5	TCGAGAGAAAATTAGGGTACCC	TCAAAATGAGGAGGCACCAAT	45.5	42.9	57.0	57.8	461 bp	1.0	47.0