Hotspot

trnL-CAA-ndhB

IRb · 99520-100096 · 577 bp

Back to Sobralia

Pi 0.0104

MarkerSeek score 64.30

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 577
variable_sites: 6
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	637 bp	1.0	80.2
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	635 bp	1.0	80.2
trnL-CAA-ndhB_p3	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.2	57.9	632 bp	1.0	78.7
trnL-CAA-ndhB_p4	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	50.0	50.0	57.7	57.9	636 bp	1.0	78.1
trnL-CAA-ndhB_p5	TCGCGTGTCTACCATTTCAC	TGCAATTGCTCAGGATACCC	50.0	50.0	58.3	57.9	634 bp	1.0	77.9