Hotspot

psbB-psbT

LSC · 77827-78458 · 632 bp

Back to Sobralia

Pi 0.0152

MarkerSeek score 64.80

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 632
variable_sites: 9
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TCAGAGATGTTTTTGCCGGT	TGGAACTTTAGGTGGTTCTCG	45.0	47.6	57.7	57.6	806-826 bp	1.0	71.1
psbB-psbT_p2	TCAGAGATGTTTTTGCCGGT	GGAACTTTAGGTGGTTCTCGA	45.0	47.6	57.7	57.3	805-825 bp	1.0	68.9
psbB-psbT_p3	GCTCAAGTGGAATTTGGAGC	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.4	57.6	768-788 bp	1.0	68.3
psbB-psbT_p4	TCAGAGATGTTTTTGCCGGT	GGAACTTTAGGTGGTTCTCGAA	45.0	45.5	57.7	58.0	805-825 bp	1.0	66.2
psbB-psbT_p5	GCTCAAGTGGAATTTGGAGC	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	57.4	57.3	767-787 bp	1.0	66.1