Hotspot

trnP-UGG-psaJ

LSC · 69720-70105 · 386 bp

Back to Sobralia

Pi 0.0214

MarkerSeek score 69.10

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 8
pi: 0.0214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	CCTGCTAAAGACCCAAACCA	50.0	50.0	58.1	57.7	501-512 bp	1.0	79.9
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	CCTGCTAAAGACCCAAACCA	50.0	50.0	58.1	57.7	500-511 bp	1.0	79.9
trnP-UGG-psaJ_p3	ACCCGTGACATTTTGTACCC	ACCTGCTAAAGACCCAAACC	50.0	50.0	58.1	57.7	502-513 bp	1.0	79.9
trnP-UGG-psaJ_p4	CCCGTGACATTTTGTACCCA	ACCTGCTAAAGACCCAAACC	50.0	50.0	58.1	57.7	501-512 bp	1.0	79.9
trnP-UGG-psaJ_p5	ACAGGATTTGAACCCGTGAC	CCTGCTAAAGACCCAAACCA	50.0	50.0	58.1	57.7	512-523 bp	1.0	79.8