Hotspot

petA-psbJ

LSC · 65613-66652 · 1040 bp

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Pi 0.0192

MarkerSeek score 69.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1040
variable_sites: 20
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	TATTCCGGGTTGGGTTCATC	50.0	50.0	58.0	57.3	1152-1162 bp	1.0	75.6
petA-psbJ_p2	GGCATCTGTGATTTTGGCAC	CTATTCCGGGTTGGGTTCAT	50.0	50.0	58.0	57.3	1153-1163 bp	1.0	75.5
petA-psbJ_p3	GAGTCCAAGGCCTTCTGTTC	TATTCCGGGTTGGGTTCATC	55.0	50.0	58.2	57.3	1177-1187 bp	1.0	74.1
petA-psbJ_p4	GAGTCCAAGGCCTTCTGTTC	CTATTCCGGGTTGGGTTCAT	55.0	50.0	58.2	57.3	1178-1188 bp	1.0	74.0
petA-psbJ_p5	GTCCAAGGCCTTCTGTTCTT	TATTCCGGGTTGGGTTCATC	50.0	50.0	57.7	57.3	1175-1185 bp	1.0	73.4