Hotspot

rbcL-accD

LSC · 58760-59594 · 835 bp

Back to Sobralia

Pi 0.0384

MarkerSeek score 75.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 32
pi: 0.0384
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGAACCAGTGGATAAGGTGG	CCACTGGTATGTCCAAGAGC	55.0	55.0	58.0	58.0	979 bp	1.0	82.2
rbcL-accD_p2	CTAGCAAATGGAGCCCTGAG	CCACTGGTATGTCCAAGAGC	55.0	55.0	58.0	58.0	1043 bp	1.0	81.9
rbcL-accD_p3	GCCGCTGCTTGTGAAATATG	CCACTGGTATGTCCAAGAGC	50.0	55.0	58.2	58.0	1020 bp	1.0	80.9
rbcL-accD_p4	TGAAGCTAGCAAATGGAGCC	CCACTGGTATGTCCAAGAGC	50.0	55.0	58.2	58.0	1048 bp	1.0	80.2
rbcL-accD_p5	CCGCTGCTTGTGAAATATGG	CCACTGGTATGTCCAAGAGC	50.0	55.0	57.5	58.0	1019 bp	1.0	78.4