Hotspot

ndhF

SSC · 112791-115010 · 2220 bp

Pi 0.00774

MarkerSeek score 50.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 69
pi: 0.00774
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCGGGTGAATGGAAAGGAAA	AGAATTGGTGAGTCGGAAACT	50.0	42.9	58.1	57.2	2604-2652 bp	1.0	70.6
ndhF_p2	AGGGGGATCCGGACAAAATA	AGAATTGGTGAGTCGGAAACT	50.0	42.9	58.1	57.2	2641-2689 bp	1.0	70.4
ndhF_p3	AAGAGATCCGGGTGAATGGA	AGAATTGGTGAGTCGGAAACT	50.0	42.9	58.1	57.2	2611-2659 bp	1.0	70.3
ndhF_p4	AGAGATCCGGGTGAATGGAA	AGAATTGGTGAGTCGGAAACT	50.0	42.9	58.1	57.2	2610-2658 bp	1.0	70.3
ndhF_p5	AAGTGAGGAAGAAAGCGACG	AGAATTGGTGAGTCGGAAACT	50.0	42.9	58.2	57.2	2745-2793 bp	1.0	69.3