Hotspot

rpl32-trnL-UAG

SSC · 116328-117092 · 765 bp

Back to Smilax

Pi 0.0193
MarkerSeek score 73.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
765
variable_sites
49
pi
0.0193
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CTAAATCGGTTGCCACTGGA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 857-907 bp 1.0 86.3
rpl32-trnL-UAG_p2 CTAAATCGGTTGCCACTGGA CAGCGTGTCTACCGATTTCA 50.0 50.0 57.9 58.0 858-908 bp 1.0 86.3
rpl32-trnL-UAG_p3 TAAATCGGTTGCCACTGGAC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 856-906 bp 1.0 85.7
rpl32-trnL-UAG_p4 TAAATCGGTTGCCACTGGAC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 857-907 bp 1.0 85.7
rpl32-trnL-UAG_p5 CTAAATCGGTTGCCACTGGA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.9 57.9 867-917 bp 1.0 85.3