Hotspot

ndhF-rpl32

SSC · 115011-116153 · 1143 bp

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Pi 0.0391
MarkerSeek score 75.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1143
variable_sites
109
pi
0.0391
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.85
markerseek_score
75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGGGTATTATCCATGCGTATTGA GAAAGCGCTTTTTCTCCTGT 39.1 45.0 57.2 56.9 1008-1278 bp 0.9 48.9
ndhF-rpl32_p2 AGGGTATTATCCATGCGTATTGA AGTGGCAACCGATTTAGCTAA 39.1 42.9 57.2 57.7 1030-1300 bp 0.9 47.6
ndhF-rpl32_p3 AGGGTATTATCCATGCGTATTGA GAAAGCGCTTTTTCTCCTGTT 39.1 42.9 57.2 57.6 1008-1278 bp 0.9 47.5
ndhF-rpl32_p4 AGGGTATTATCCATGCGTATTGA AGAAAGCGCTTTTTCTCCTG 39.1 45.0 57.2 56.6 1009-1279 bp 0.9 47.5
ndhF-rpl32_p5 AGGGTATTATCCATGCGTATTGA AGTGGCAACCGATTTAGCTA 39.1 45.0 57.2 56.9 1030-1300 bp 0.8 43.9