Hotspot

rpl22-rps19

IRb · 85602-85775 · 174 bp

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Pi 0.0672
MarkerSeek score 87.50
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
IRb
length_bp
174
variable_sites
34
pi
0.0672
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.82
markerseek_score
87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 GCCCGCGAATTTGATCAAC CGCCTACTCTAACTTTCGCA 52.6 50.0 58.1 57.7 306-508 bp 1.0 81.4
rpl22-rps19_p2 CCCGCGAATTTGATCAACTAC CGCCTACTCTAACTTTCGCA 47.6 50.0 57.7 57.7 305-507 bp 1.0 79.3
rpl22-rps19_p3 GCCCGCGAATTTGATCAAC TCGCGCCTACTCTAACTTTC 52.6 50.0 58.1 57.4 309-511 bp 1.0 79.1
rpl22-rps19_p4 GCCCGCGAATTTGATCAACT CGCCTACTCTAACTTTCGCA 50.0 50.0 59.5 57.7 306-508 bp 1.0 77.4
rpl22-rps19_p5 GCCCGCGAATTTGATCAAC CGCGCCTACTCTAACTTTCG 52.6 55.0 58.1 58.8 308-510 bp 1.0 77.1