Hotspot

rpl33-rps18

LSC · 69561-69949 · 389 bp

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Pi 0.0384
MarkerSeek score 74.80
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
389
variable_sites
43
pi
0.0384
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.94
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 GCACAATACACCCAGTCGAT TGGATTTACGAAAAGGTTGCT 50.0 38.1 58.0 56.1 431-521 bp 1.0 68.1
rpl33-rps18_p2 AGAATCGGCACAATACACCC TGGATTTACGAAAAGGTTGCT 50.0 38.1 58.0 56.1 438-528 bp 1.0 67.9
rpl33-rps18_p3 TGTCGCTATTGTCACAAGCA TGGATTTACGAAAAGGTTGCT 45.0 38.1 57.8 56.1 394-484 bp 1.0 67.3
rpl33-rps18_p4 AATCGGCACAATACACCCAG TGGATTTACGAAAAGGTTGCT 50.0 38.1 58.2 56.1 436-526 bp 1.0 66.3
rpl33-rps18_p5 GAATCGGCACAATACACCCA TGGATTTACGAAAAGGTTGCT 50.0 38.1 58.3 56.1 437-527 bp 1.0 66.2