Hotspot

psaJ-rpl33

LSC · 68901-69356 · 456 bp

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Pi 0.0388
MarkerSeek score 90.80
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
456
variable_sites
63
pi
0.0388
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.98
markerseek_score
90.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 CGTTTATTCCCAGACGCCTT ACACCATTTCGGACACAACA 50.0 45.0 58.3 58.0 563-582 bp 1.0 89.5
psaJ-rpl33_p2 ACGCCTTGTCATTTCCCTTT ACACCATTTCGGACACAACA 45.0 45.0 58.0 58.0 550-569 bp 0.9 87.2
psaJ-rpl33_p3 ATCGTTTATTCCCAGACGCC ACACCATTTCGGACACAACA 50.0 45.0 58.1 58.0 565-584 bp 0.9 86.6
psaJ-rpl33_p4 ACGCCTTGTCATTTCCCTTT TGACACCATTTCGGACACAA 45.0 45.0 58.0 57.7 552-571 bp 0.9 84.9
psaJ-rpl33_p5 ACGCCTTGTCATTTCCCTTT TTGACACCATTTCGGACACA 45.0 45.0 58.0 57.7 553-572 bp 0.9 84.9