Hotspot

petA-psbJ

LSC · 64356-65642 · 1287 bp

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Pi 0.0342
MarkerSeek score 89.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1287
variable_sites
124
pi
0.0342
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.95
markerseek_score
89.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTGGC CCGGATTGGGTTCATCTCTG 50.0 55.0 57.8 58.0 1258-1492 bp 1.0 89.8
petA-psbJ_p2 GGCCTTTTGTTCTTCTTGGC TCCGGATTGGGTTCATCTCT 50.0 50.0 57.8 58.1 1259-1493 bp 1.0 89.1
petA-psbJ_p3 CGTCCAAGGCCTTTTGTTCT CCGGATTGGGTTCATCTCTG 50.0 55.0 58.7 58.0 1265-1499 bp 1.0 85.5
petA-psbJ_p4 GGCCTTTTGTTCTTCTTGGC AACCGGTATTCCTGTGATCG 50.0 50.0 57.8 57.4 1311-1545 bp 1.0 85.1
petA-psbJ_p5 GGCCTTTTGTTCTTCTTGGC CGGTATTCCTGTGATCGGTT 50.0 50.0 57.8 57.4 1308-1542 bp 1.0 85.1