Hotspot

accD-psaI

LSC · 59659-60702 · 1044 bp

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Pi 0.0356
MarkerSeek score 77.20
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
1044
variable_sites
117
pi
0.0356
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.93
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TCACAAGCGGCTGAGTATTC TTGTTTTTCGAGACCCCGTT 50.0 45.0 58.0 58.0 1659-1781 bp 1.0 90.3
accD-psaI_p2 TCACAAGCGGCTGAGTATTC TTCCACGCAATTGTACACCA 50.0 45.0 58.0 58.0 1861-1983 bp 1.0 89.9
accD-psaI_p3 TCACAAGCGGCTGAGTATTC AATAGACCCGCGATCCCATA 50.0 50.0 58.0 58.1 1825-1947 bp 1.0 89.7
accD-psaI_p4 GCGAGAAAATCACCCGTTTG TTCCACGCAATTGTACACCA 50.0 45.0 58.0 58.0 2181-2303 bp 1.0 89.2
accD-psaI_p5 TCACAAGCGGCTGAGTATTC CGTATGCATATCCGTCGTGT 50.0 50.0 58.0 58.0 1407 bp 0.1 43.5