Hotspot

atpI-rps2

LSC · 15352-15721 · 370 bp

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Pi 0.036
MarkerSeek score 85.40
Primer available yes
Label atpI-rps2

Feature Metadata

label_name
atpI-rps2
feature_type
igs
region
LSC
length_bp
370
variable_sites
45
pi
0.036
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.92
markerseek_score
85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-rps2_p1 GTTGGCCTACTTCTACACCC ATTTCGATTCCAGCCAACGA 55.0 45.0 57.6 57.9 509-584 bp 1.0 86.9
atpI-rps2_p2 GTTGGCCTACTTCTACACCC CAGCCAACGATGACGCTATA 55.0 50.0 57.6 57.9 499-574 bp 1.0 86.8
atpI-rps2_p3 GTTGGCCTACTTCTACACCC ATTTGCGAGGGTCGTTCTAG 55.0 50.0 57.6 57.7 440-515 bp 1.0 86.3
atpI-rps2_p4 GTTGGCCTACTTCTACACCC TTTCGATTCCAGCCAACGAT 55.0 45.0 57.6 57.9 508-583 bp 0.9 82.3
atpI-rps2_p5 GGCCTACTTCTACACCCGAT TTTCGATTCCAGCCAACGAT 55.0 45.0 58.6 57.9 505-580 bp 0.9 80.8