Hotspot

trnG-UCC-trnR-UCU

LSC · 9752-9971 · 220 bp

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Pi 0.0309
MarkerSeek score 73.60
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
220
variable_sites
18
pi
0.0309
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.86
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 250-300 bp 1.0 72.7
trnG-UCC-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.1 56.9 253-303 bp 1.0 72.4
trnG-UCC-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 256-306 bp 1.0 71.9
trnG-UCC-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 55.0 45.5 58.1 57.9 259-309 bp 1.0 71.6
trnG-UCC-trnR-UCU_p5 CCAACGTCGACTATAACCCC AGAAGACCTCTGTCCTATCCA 55.0 47.6 57.8 56.9 271-321 bp 1.0 70.8