Hotspot

rps16-trnQ-UUG

LSC · 5860-6542 · 683 bp

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Pi 0.0218
MarkerSeek score 76.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
683
variable_sites
52
pi
0.0218
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 721-767 bp 1.0 87.8
rps16-trnQ-UUG_p2 TCCACAGTCGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 743-789 bp 1.0 87.6
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 732-778 bp 1.0 87.2
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 725-771 bp 1.0 86.7
rps16-trnQ-UUG_p5 TCCACAGTCGATCATGTCCT CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.1 57.8 747-793 bp 1.0 86.5