Hotspot

ndhE-ndhG

SSC · 118114-118367 · 254 bp

Back to Smelowskia

Pi 0.0489

MarkerSeek score 78.50

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 254
variable_sites: 23
pi: 0.0489
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GGCACTCAAAACAAGTACATGT	TAGGTGCAATTTCTGTGGCT	40.9	45.0	57.4	57.4	313-318 bp	1.0	62.1
ndhE-ndhG_p2	GGCACTCAAAACAAGTACATGT	AGGTGCAATTTCTGTGGCTC	40.9	50.0	57.4	58.7	312-317 bp	1.0	60.8
ndhE-ndhG_p3	AGGCACTCAAAACAAGTACATG	TAGGTGCAATTTCTGTGGCT	40.9	45.0	57.2	57.4	314-319 bp	1.0	60.0
ndhE-ndhG_p4	AGGCACTCAAAACAAGTACA	TAGGTGCAATTTCTGTGGCT	40.0	45.0	55.1	57.4	314-319 bp	1.0	59.6
ndhE-ndhG_p5	AGGCACTCAAAACAAGTACATG	AGGTGCAATTTCTGTGGCTC	40.9	50.0	57.2	58.7	313-318 bp	1.0	58.7