Hotspot

ndhF-rpl32

SSC · 112694-113581 · 888 bp

Back to Smelowskia

Pi 0.0631

MarkerSeek score 85.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 888
variable_sites: 100
pi: 0.0631
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCAATAGGTCGTGTGAACCA	AGCGGCAACTTGTTCAAAAC	50.0	45.0	58.2	58.1	1947-2107 bp	1.0	88.5
ndhF-rpl32_p2	TGCAATAGGTCGTGTGAACC	AGCGGCAACTTGTTCAAAAC	50.0	45.0	58.2	58.1	1948-2108 bp	1.0	88.5
ndhF-rpl32_p3	GGTCGTGTGAACCAAAAACC	AGCGGCAACTTGTTCAAAAC	50.0	45.0	57.8	58.1	1941-2101 bp	1.0	88.5
ndhF-rpl32_p4	GCAATAGGTCGTGTGAACCA	TTTCACCATAGCGGCAACTT	50.0	45.0	58.2	58.1	1956-2116 bp	1.0	88.4
ndhF-rpl32_p5	TGCAATAGGTCGTGTGAACC	TTTCACCATAGCGGCAACTT	50.0	45.0	58.2	58.1	1957-2117 bp	1.0	88.3