Hotspot

trnF-UUC-ndhJ

LSC · 47935-48517 · 583 bp

Back to Smelowskia

Pi 0.0274

MarkerSeek score 69.10

Primer available yes

Label trnF-UUC-ndhJ

Feature Metadata

label_name: trnF-UUC-ndhJ
feature_type: igs
region: LSC
length_bp: 583
variable_sites: 29
pi: 0.0274
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-UUC-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CATCCACGACTGAAACGGAT	50.0	50.0	58.1	58.0	714-754 bp	1.0	84.3
trnF-UUC-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	687-727 bp	1.0	84.3
trnF-UUC-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	686-726 bp	1.0	84.3
trnF-UUC-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CCTAATGCCCGAAAGTTGGA	50.0	50.0	58.1	57.9	694-734 bp	1.0	83.5
trnF-UUC-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TCCTAATGCCCGAAAGTTGG	50.0	50.0	58.1	57.9	695-735 bp	1.0	83.5