Hotspot

trnD-GAC-trnY-UAC

LSC · 29826-30257 · 432 bp

Back to Smelowskia

Pi 0.0432

MarkerSeek score 77.90

Primer available yes

Label trnD-GAC-trnY-UAC

Feature Metadata

label_name: trnD-GAC-trnY-UAC
feature_type: igs
region: LSC
length_bp: 432
variable_sites: 34
pi: 0.0432
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GAC-trnY-UAC_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	502-508 bp	1.0	80.0
trnD-GAC-trnY-UAC_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	501-507 bp	1.0	80.0
trnD-GAC-trnY-UAC_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	523-529 bp	1.0	78.8
trnD-GAC-trnY-UAC_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	522-528 bp	1.0	78.7
trnD-GAC-trnY-UAC_p5	CGGTGCTCTGACCAATTGAA	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.5	58.4	556-562 bp	1.0	78.0