Hotspot

trnK-AAA-rps16

LSC · 4307-4884 · 578 bp

Back to Smelowskia

Pi 0.0421
MarkerSeek score 80.10
Primer available yes
Label trnK-AAA-rps16

Feature Metadata

label_name
trnK-AAA-rps16
feature_type
igs
region
LSC
length_bp
578
variable_sites
43
pi
0.0421
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-AAA-rps16_p1 TACCGTTGAGTTAGCAACCC ACGGAATTAAGTCTTAATAAAACGA 50.0 28.0 57.5 55.1 634-764 bp 0.75 37.4
trnK-AAA-rps16_p2 AAAGCCGAGTACTCTACCGT ACGGAATTAAGTCTTAATAAAACGA 50.0 28.0 58.2 55.1 648-778 bp 0.75 37.3
trnK-AAA-rps16_p3 AAGCCGAGTACTCTACCGTT ACGGAATTAAGTCTTAATAAAACGA 50.0 28.0 58.2 55.1 647-777 bp 0.75 37.3
trnK-AAA-rps16_p4 AACTCTACCGCAGATCTGGA ACGGAATTAAGTCTTAATAAAACGA 50.0 28.0 57.9 55.1 716-846 bp 0.75 37.1
trnK-AAA-rps16_p5 AACTCTACCGCAGATCTGGA ACGGAATTAAGTCTTAATAAAACGAA 50.0 26.9 57.9 55.8 716-846 bp 0.75 37.1