Hotspot

ycf3

LSC · 45139-47085 · 1947 bp

Back to Sloanea

Pi 0.000823

MarkerSeek score 59.00

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1947
variable_sites: 3
pi: 0.000823
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	ACAAATGGGTTACGGGTTATTCT	TCCTTTGTTTGTGGGATCGT	39.1	45.0	58.1	57.4	2107-2119 bp	1.0	50.8
ycf3_p2	AACAAATGGGTTACGGGTTA	TCCTTTGTTTGTGGGATCGT	40.0	45.0	54.8	57.4	2108-2120 bp	1.0	50.2
ycf3_p3	TAACAAATGGGTTACGGGTT	TCCTTTGTTTGTGGGATCGT	40.0	45.0	54.8	57.4	2109-2121 bp	1.0	50.2
ycf3_p4	ACAAATGGGTTACGGGTTATTC	TCCTTTGTTTGTGGGATCGT	40.9	45.0	56.7	57.4	2107-2119 bp	1.0	48.8
ycf3_p5	ACAAATGGGTTACGGGTTAT	TCCTTTGTTTGTGGGATCGT	40.0	45.0	54.6	57.4	2107-2119 bp	1.0	48.2