Hotspot

ycf1

SSC · 127466-133273 · 5808 bp

Back to Sloanea

Pi 0.00148

MarkerSeek score 53.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5808
variable_sites: 18
pi: 0.00148
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTAATGAGTCTGCTTCAAA	CGTCCTTCAATATCACGGAAC	35.0	47.6	52.1	56.8	324 bp	1.0	41.0
ycf1_p2	AGTAATGAGTCTGCTTCAAAA	CGTCCTTCAATATCACGGAAC	33.3	47.6	53.1	56.8	324 bp	1.0	41.0
ycf1_p3	AGTAATGAGTCTGCTTCAAA	AGATCCGTCCTTCAATATCACG	35.0	45.5	52.1	57.8	329 bp	1.0	41.0
ycf1_p4	AGTAATGAGTCTGCTTCAAAA	AGATCCGTCCTTCAATATCACG	33.3	45.5	53.1	57.8	329 bp	1.0	41.0
ycf1_p5	TGATTTAGTAATGAGTCTGCTTCA	CGTCCTTCAATATCACGGAAC	33.3	47.6	55.9	56.8	330 bp	1.0	41.0