Hotspot

rps8

LSC · 84231-84632 · 402 bp

Back to Sloanea

Pi 0.000995

MarkerSeek score 25.80

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 402
variable_sites: 1
pi: 0.000995
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	TGCCGTTGGAAAGTGATTCA	CAACCCCAGACCCATTTGAA	45.0	50.0	58.0	58.0	1144-1159 bp	1.0	81.3
rps8_p2	TGCCGTTGGAAAGTGATTCA	TTCAACCCCAGACCCATTTG	45.0	50.0	58.0	58.0	1146-1161 bp	1.0	81.3
rps8_p3	ATCAAAACGAACCCGGAACA	CAACCCCAGACCCATTTGAA	45.0	50.0	58.0	58.0	1164-1179 bp	1.0	81.3
rps8_p4	TCAAAACGAACCCGGAACAT	CAACCCCAGACCCATTTGAA	45.0	50.0	58.0	58.0	1163-1178 bp	1.0	81.3
rps8_p5	TCAAAACGAACCCGGAACAT	TTCAACCCCAGACCCATTTG	45.0	50.0	58.0	58.0	1165-1180 bp	1.0	81.3