Hotspot

psbF-psbE

LSC · 68141-68149 · 9 bp

Back to Sloanea

Pi 0.0444
MarkerSeek score 70.00
Primer available yes
Label psbF-psbE

Feature Metadata

label_name
psbF-psbE
feature_type
igs
region
LSC
length_bp
9
variable_sites
1
pi
0.0444
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbF-psbE_p1 ACGGTAGGTACAGCTAGTCC GAGCCGACAAGGAATTCCAT 55.0 50.0 58.0 58.0 162 bp 1.0 79.7
psbF-psbE_p2 GGTAGGTACAGCTAGTCCGT GAGCCGACAAGGAATTCCAT 55.0 50.0 58.0 58.0 160 bp 1.0 79.7
psbF-psbE_p3 ACGGTAGGTACAGCTAGTCC TACAGAGAGCCGACAAGGAA 55.0 50.0 58.0 58.1 168 bp 1.0 79.4
psbF-psbE_p4 GGTAGGTACAGCTAGTCCGT TACAGAGAGCCGACAAGGAA 55.0 50.0 58.0 58.1 166 bp 1.0 79.4
psbF-psbE_p5 GGTAGGTACAGCTAGTCCGT TTACAGAGAGCCGACAAGGA 55.0 50.0 58.0 58.1 167 bp 1.0 79.4