Hotspot

ycf4-cemA

LSC · 63777-64662 · 886 bp

Back to Sloanea

Pi 0.00158

MarkerSeek score 52.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 886
variable_sites: 3
pi: 0.00158
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	GAGAGAGAGATCCACCAGGG	45.0	60.0	57.9	58.3	988-990 bp	1.0	77.3
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	GAGAGAGAGATCCACCAGGG	50.0	60.0	58.1	58.3	990-992 bp	1.0	77.0
ycf4-cemA_p3	TGCGCGTACCAATTGAAGTA	TGAGAGAGAGATCCACCAGG	45.0	55.0	57.9	57.5	989-991 bp	1.0	76.1
ycf4-cemA_p4	CTTGCGCGTACCAATTGAAG	TGAGAGAGAGATCCACCAGG	50.0	55.0	58.1	57.5	991-993 bp	1.0	75.8
ycf4-cemA_p5	TTTCTTGCGCGTACCAATTG	GAGAGAGAGATCCACCAGGG	45.0	60.0	57.7	58.3	993-995 bp	1.0	75.4