Hotspot

atpB-rbcL

LSC · 57433-58208 · 776 bp

Back to Sloanea

Pi 0.00129
MarkerSeek score 52.40
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
776
variable_sites
2
pi
0.00129
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 TGAGCGATACGTCCTAGGTT GCCTTGAATCCAACACTTGC 50.0 50.0 58.0 57.9 892-894 bp 1.0 79.4
atpB-rbcL_p2 TTGAGCGATACGTCCTAGGT GCCTTGAATCCAACACTTGC 50.0 50.0 58.0 57.9 893-895 bp 1.0 79.4
atpB-rbcL_p3 TACCGGGCCAATGATTTGAG GCCTTGAATCCAACACTTGC 50.0 50.0 57.9 57.9 908-910 bp 1.0 79.4
atpB-rbcL_p4 CTGGGGGAAAAGCTACATCC GCCTTGAATCCAACACTTGC 55.0 50.0 57.9 57.9 930-932 bp 1.0 79.3
atpB-rbcL_p5 GGGGGAAAAGCTACATCCAG GCCTTGAATCCAACACTTGC 55.0 50.0 57.9 57.9 928-930 bp 1.0 79.3