Hotspot

psbZ-trnG-GCC

LSC · 38663-39098 · 436 bp

Back to Sloanea

Pi 0.00229

MarkerSeek score 57.70

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 436
variable_sites: 2
pi: 0.00229
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	AATCGAACCCGCATCTTCTC	55.0	50.0	58.0	58.1	600-643 bp	1.0	80.0
psbZ-trnG-GCC_p2	CTTCTCCTGATGGTTGGTCG	GAATCGAACCCGCATCTTCT	55.0	50.0	58.0	58.1	601-644 bp	1.0	80.0
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	603-646 bp	1.0	78.5
psbZ-trnG-GCC_p4	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	604-647 bp	1.0	78.5
psbZ-trnG-GCC_p5	TCCTGATGGTTGGTCGAGTA	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	596-639 bp	1.0	78.4