Hotspot

trnC-GCA-petN

LSC · 29540-30453 · 914 bp

Back to Sloanea

Pi 0.00372

MarkerSeek score 39.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 914
variable_sites: 7
pi: 0.00372
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TACCATTAAAGCAGCCCAGG	50.0	50.0	58.1	57.6	982 bp	1.0	76.4
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	TACCATTAAAGCAGCCCAGG	50.0	50.0	58.4	57.6	1000 bp	1.0	74.2
trnC-GCA-petN_p3	CCAATCCAAGCAAAAGGGGA	TACCATTAAAGCAGCCCAGG	50.0	50.0	58.4	57.6	1071 bp	1.0	74.2
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	GCCCAGGCGAGATTTACTAT	50.0	50.0	58.1	57.2	969 bp	1.0	73.2
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	GACTACCATTAAAGCAGCCCA	50.0	47.6	58.1	58.0	985 bp	1.0	71.9