Hotspot

rps16-trnQ-UUG

LSC · 6784-7404 · 621 bp

Back to Sloanea

Pi 0.00225

MarkerSeek score 52.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 621
variable_sites: 3
pi: 0.00225
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CAGCGGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	712-727 bp	1.0	79.5
rps16-trnQ-UUG_p2	GCGGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	710-725 bp	1.0	79.5
rps16-trnQ-UUG_p3	ACACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.7	58.1	689-704 bp	1.0	77.2
rps16-trnQ-UUG_p4	AGCGGATCATGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	711-726 bp	1.0	77.1
rps16-trnQ-UUG_p5	CCAAGAGCACCCTCATTTCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	764-779 bp	1.0	75.7