Hotspot

trnK-UUU-rps16

LSC · 4571-5592 · 1022 bp

Back to Sloanea

Pi 0.00508
MarkerSeek score 66.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1022
variable_sites
7
pi
0.00508
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 1060-1156 bp 1.0 83.0
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 1059-1155 bp 1.0 83.0
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TATTTCAAAGAAGGCGGGGG 50.0 50.0 58.2 57.9 1026-1122 bp 1.0 82.1
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TATTTCAAAGAAGGCGGGGG 50.0 50.0 58.2 57.9 1025-1121 bp 1.0 82.1
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 1069-1165 bp 1.0 82.0