Hotspot

trnH-GUG-psbA

LSC · 145-612 · 468 bp

Back to Sloanea

Pi 0.00342

MarkerSeek score 40.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 468
variable_sites: 3
pi: 0.00342
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	55.0	55.0	58.1	58.3	586 bp	1.0	76.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	600 bp	1.0	76.6
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	599 bp	1.0	76.6
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	601 bp	1.0	76.6
trnH-GUG-psbA_p5	TATTTATGGGCGAACGACGG	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.1	58.3	647 bp	1.0	76.5