Hotspot

ycf46

Genome · 59533-61026 · 1494 bp

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Pi 0.0681

MarkerSeek score 79.20

Primer available yes

Label ycf46

Feature Metadata

label_name: ycf46
feature_type: gene
region: Genome
length_bp: 1494
variable_sites: 204
pi: 0.0681
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf46_p1	TGGAGAAGTTATGGGCAACG	GGGTGCTATTCGACCAATCA	50.0	50.0	57.9	57.7	2407 bp	0.2	44.5
ycf46_p2	TGGAGAAGTTATGGGCAACG	TGGGTGCTATTCGACCAATC	50.0	50.0	57.9	57.7	2408 bp	0.2	44.5
ycf46_p3	CGTGATGCTGTAGGTTGGAG	GGGTGCTATTCGACCAATCA	55.0	50.0	58.4	57.7	2422 bp	0.2	42.6
ycf46_p4	CGTGATGCTGTAGGTTGGAG	TGGGTGCTATTCGACCAATC	55.0	50.0	58.4	57.7	2423 bp	0.2	42.6
ycf46_p5	GGAGAAGTTATGGGCAACGA	GGGTGCTATTCGACCAATCA	50.0	50.0	57.6	57.7	2406 bp	0.2	42.2
ycf46_p1	GGGTGCTATTCGACCAATCA	TGGAGAAGTTATGGGCAACG	50.0	50.0	57.7	57.9	2407 bp	0.2	44.5
ycf46_p2	TGGGTGCTATTCGACCAATC	TGGAGAAGTTATGGGCAACG	50.0	50.0	57.7	57.9	2408 bp	0.2	44.5
ycf46_p3	GGGTGCTATTCGACCAATCA	CGTGATGCTGTAGGTTGGAG	50.0	55.0	57.7	58.4	2422 bp	0.2	42.6
ycf46_p4	TGGGTGCTATTCGACCAATC	CGTGATGCTGTAGGTTGGAG	50.0	55.0	57.7	58.4	2423 bp	0.2	42.6
ycf46_p5	GGGTGCTATTCGACCAATCA	GGAGAAGTTATGGGCAACGA	50.0	50.0	57.7	57.6	2406 bp	0.2	42.2