Hotspot

rps3

Genome · 37884-38528 · 645 bp

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Pi 0.0546

MarkerSeek score 79.30

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: Genome
length_bp: 645
variable_sites: 74
pi: 0.0546
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TATTCGACCACGTGCTCAAG	TGCACGAGCTGTAACAGTTT	50.0	45.0	58.0	58.1	1045-1047 bp	0.8	77.9
rps3_p2	TATTCGACCACGTGCTCAAG	ATGGTACGTCGAGTTGCTTC	50.0	50.0	58.0	58.0	973 bp	0.2	47.9
rps3_p3	TATTCGACCACGTGCTCAAG	CACTGCCCCTTTACCTGATC	50.0	55.0	58.0	58.0	1082 bp	0.2	47.6
rps3_p4	ATTTGCAGATGAAGGCCCAA	ATGGTACGTCGAGTTGCTTC	45.0	50.0	58.1	58.0	1003 bp	0.2	47.5
rps3_p5	CGTGGGCGTTCATATCAAGA	ATGGTACGTCGAGTTGCTTC	50.0	50.0	58.1	58.0	1152 bp	0.2	47.4