Hotspot

rpl2

Genome · 35874-36701 · 828 bp

Back to Skeletonema

Pi 0.0662

MarkerSeek score 79.30

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: Genome
length_bp: 828
variable_sites: 113
pi: 0.0662
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	AGCAGGGTCAAATCGTTCTC	TGAACTTCGTGACCAAGTTGT	50.0	42.9	57.9	58.0	1719-1721 bp	1.0	79.1
rpl2_p2	GAGCAGGGTCAAATCGTTCT	TGAACTTCGTGACCAAGTTGT	50.0	42.9	57.9	58.0	1720-1722 bp	1.0	79.1
rpl2_p3	TTGGTCCAAAGCCAAGAACA	TGAACTTCGTGACCAAGTTGT	45.0	42.9	57.8	58.0	1672-1674 bp	0.6	62.2
rpl2_p4	AGAGCAGGGTCAAATCGTTC	TGAACTTCGTGACCAAGTTGT	50.0	42.9	57.9	58.0	1721-1723 bp	0.6	60.1
rpl2_p5	TGGTCCAAAGCCAAGAACAA	TGAACTTCGTGACCAAGTTGT	45.0	42.9	57.8	58.0	1671-1672 bp	0.4	50.7