Hotspot

petF

Genome · 4575-4874 · 300 bp

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Pi 0.142

MarkerSeek score 73.70

Primer available yes

Label petF

Feature Metadata

label_name: petF
feature_type: gene
region: Genome
length_bp: 300
variable_sites: 79
pi: 0.142
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petF_p1	TCCTATGGAACGTCACTGGT	CGTTGGAGAGACAAACCAGT	50.0	50.0	58.1	57.8	1215-1235 bp	1.0	89.4
petF_p2	TCCTATGGAACGTCACTGGT	TTTGGGCACATGAACGTACA	50.0	45.0	58.1	58.0	1253-1273 bp	0.8	82.4
petF_p3	TGGACATGGGTTCAATTCCC	TTTGGGCACATGAACGTACA	50.0	45.0	57.8	58.0	1333-1353 bp	0.8	80.9
petF_p4	TACGTGGAAAAGCAAGTCGT	TTTGGGCACATGAACGTACA	45.0	45.0	57.8	58.0	809-827 bp	0.6	73.2
petF_p5	TCCTATGGAACGTCACTGGT	GGTGGAAATCCGGGTCAATT	50.0	50.0	58.1	58.2	1502 bp	0.2	46.2