Hotspot

dnaK

Genome · 32109-33905 · 1797 bp

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Pi 0.0634

MarkerSeek score 78.10

Primer available yes

Label dnaK

Feature Metadata

label_name: dnaK
feature_type: gene
region: Genome
length_bp: 1797
variable_sites: 231
pi: 0.0634
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaK_p1	TATTTCGCGTGCAATTGTCG	CATTGGGCCTCTTGCAAAAC	45.0	50.0	57.8	58.2	2998 bp	0.2	45.2
dnaK_p2	GGGATTGCTGTTATCCGTGT	GTGTAACGATACAAGGCCCA	50.0	50.0	58.0	57.6	2917 bp	0.2	44.6
dnaK_p3	GGGATTGCTGTTATCCGTGT	TGTGTAACGATACAAGGCCC	50.0	50.0	58.0	57.6	2918 bp	0.2	44.6
dnaK_p4	TATTTCGCGTGCAATTGTCG	ATTGGGCCTCTTGCAAAACT	45.0	45.0	57.8	58.3	2997 bp	0.2	44.5
dnaK_p5	TATTTCGCGTGCAATTGTCG	TCATTGGGCCTCTTGCAAAA	45.0	45.0	57.8	58.3	2999 bp	0.2	44.5