Hotspot

trnV(uac)-psbH

Genome · 117781-117997 · 217 bp

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Pi 0.159
MarkerSeek score 79.30
Primer available yes
Label trnV(uac)-psbH

Feature Metadata

label_name
trnV(uac)-psbH
feature_type
igs
region
Genome
length_bp
217
variable_sites
59
pi
0.159
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.91
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV(uac)-psbH_p1 AACCTGTGACCTTCTGCTTG AGATGTTGATTGGGCAAATGC 50.0 42.9 58.0 57.7 246-299 bp 1.0 81.7
trnV(uac)-psbH_p2 AACCTGTGACCTTCTGCTTG GATGTTGATTGGGCAAATGCT 50.0 42.9 58.0 57.7 245-298 bp 1.0 81.7
trnV(uac)-psbH_p3 TTGAACCTGTGACCTTCTGC AGATGTTGATTGGGCAAATGC 50.0 42.9 58.0 57.7 249-302 bp 1.0 81.7
trnV(uac)-psbH_p4 TTGAACCTGTGACCTTCTGC GATGTTGATTGGGCAAATGCT 50.0 42.9 58.0 57.7 248-301 bp 1.0 81.7
trnV(uac)-psbH_p5 AGACACTCTACCGCTGAGTT GATGTTGATTGGGCAAATGCT 50.0 42.9 58.1 57.7 219-272 bp 1.0 81.3