Hotspot

rps20-rpoB

Genome · 98664-99108 · 445 bp

Back to Skeletonema

Pi 0.157

MarkerSeek score 80.90

Primer available yes

Label rps20-rpoB

Feature Metadata

label_name: rps20-rpoB
feature_type: igs
region: Genome
length_bp: 445
variable_sites: 137
pi: 0.157
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps20-rpoB_p1	GGCAGAACGCTTATTTGCAT	GCTTCTCCTTGCCAATTCCA	45.0	50.0	57.4	58.5	1608-1619 bp	0.4	53.0
rps20-rpoB_p2	GGCAGAACGCTTATTTGCAT	AACACGTTCACACCCATTGA	45.0	45.0	57.4	58.0	1351 bp	0.2	43.1
rps20-rpoB_p3	CAAATACCGTGAAACACCGG	AACACGTTCACACCCATTGA	50.0	45.0	57.4	58.0	1369 bp	0.2	42.8
rps20-rpoB_p4	GGCAGAACGCTTATTTGCAT	ACACGTTCACACCCATTGAT	45.0	45.0	57.4	57.7	1350 bp	0.2	41.3
rps20-rpoB_p5	CAAATACCGTGAAACACCGG	ACACGTTCACACCCATTGAT	50.0	45.0	57.4	57.7	1368 bp	0.2	41.0