Hotspot

chlI-petA

Genome · 92933-93313 · 381 bp

Back to Skeletonema

Pi 0.234

MarkerSeek score 78.90

Primer available yes

Label chlI-petA

Feature Metadata

label_name: chlI-petA
feature_type: igs
region: Genome
length_bp: 381
variable_sites: 170
pi: 0.234
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlI-petA_p1	CTGAAGACCGTGTTTGTGGA	TGGTGGTTTTGGTCAAGAGG	50.0	50.0	58.1	57.9	1263-1277 bp	0.4	61.8
chlI-petA_p2	TCCGTACAGTGAAAGACCCT	TGGTGGTTTTGGTCAAGAGG	50.0	50.0	58.0	57.9	960 bp	0.2	47.4
chlI-petA_p3	CCAGGCTTATTGGCAAAAGC	TGGTGGTTTTGGTCAAGAGG	50.0	50.0	58.0	57.9	1195 bp	0.2	47.4
chlI-petA_p4	TCCGTACAGTGAAAGACCCT	GTTGTTGCACCTGATCCAGA	50.0	50.0	58.0	58.1	1268 bp	0.2	47.1
chlI-petA_p5	CACAGTTGAACGTGAAGGGA	TGGTGGTTTTGGTCAAGAGG	50.0	50.0	58.1	57.9	1085 bp	0.2	47.1