Hotspot

ycf33-trnT(ugu)

Genome · 88649-88928 · 280 bp

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Pi 0.233

MarkerSeek score 77.50

Primer available yes

Label ycf33-trnT(ugu)

Feature Metadata

label_name: ycf33-trnT(ugu)
feature_type: igs
region: Genome
length_bp: 280
variable_sites: 124
pi: 0.233
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf33-trnT(ugu)_p1	AGACTCGAACTCGCAACTTC	ACGACCATATGTCATACGCG	50.0	50.0	57.9	58.0	1384 bp	0.2	47.2
ycf33-trnT(ugu)_p2	GAGACTCGAACTCGCAACTT	ACGACCATATGTCATACGCG	50.0	50.0	57.9	58.0	1385 bp	0.2	47.2
ycf33-trnT(ugu)_p3	CGACCTTGGGCTTATGAGTC	ACGACCATATGTCATACGCG	55.0	50.0	58.1	58.0	1110 bp	0.2	47.2
ycf33-trnT(ugu)_p4	AGACTCGAACTCGCAACTTC	TTGAGAAGCTCCTACCGGAA	50.0	50.0	57.9	58.1	1528 bp	0.2	47.0
ycf33-trnT(ugu)_p5	GAGACTCGAACTCGCAACTT	TTGAGAAGCTCCTACCGGAA	50.0	50.0	57.9	58.1	1529 bp	0.2	47.0