Hotspot

psaF-psbI

Genome · 73791-74018 · 228 bp

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Pi 0.088

MarkerSeek score 79.20

Primer available yes

Label psaF-psbI

Feature Metadata

label_name: psaF-psbI
feature_type: igs
region: Genome
length_bp: 228
variable_sites: 36
pi: 0.088
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaF-psbI_p1	ACCAACCCATCCAATCCAAC	CCCTAATCGTCGTGACCTTG	50.0	55.0	58.1	58.1	624-632 bp	0.6	69.6
psaF-psbI_p2	CAACCCATCCAATCCAACCA	CCCTAATCGTCGTGACCTTG	50.0	55.0	58.1	58.1	622-630 bp	0.6	69.6
psaF-psbI_p3	GCTAAAGCTGGGGGAGAATC	CCCTAATCGTCGTGACCTTG	55.0	55.0	58.0	58.1	447-451 bp	0.4	59.8
psaF-psbI_p4	GCTAAAGCTGGGGGAGAATC	CCAACTTTCAGTTTGGGGGA	55.0	50.0	58.0	57.9	834 bp	0.2	47.1
psaF-psbI_p5	GCTAAAGCTGGGGGAGAATC	TCCAACTTTCAGTTTGGGGG	55.0	50.0	58.0	57.9	835 bp	0.2	47.1