Hotspot

trnQ(uug)-trnR(acg)

Genome · 29994-30060 · 67 bp

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Pi 0.147

MarkerSeek score 83.30

Primer available yes

Label trnQ(uug)-trnR(acg)

Feature Metadata

label_name: trnQ(uug)-trnR(acg)
feature_type: igs
region: Genome
length_bp: 67
variable_sites: 19
pi: 0.147
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ(uug)-trnR(acg)_p1	GAGTCAAAGTCCACAGCCTT	GGGGTTCGAATCCCTTCTTG	50.0	55.0	57.7	58.2	129-141 bp	1.0	87.9
trnQ(uug)-trnR(acg)_p2	CAAAGTCCACAGCCTTACCA	GGGGTTCGAATCCCTTCTTG	50.0	55.0	57.7	58.2	125-137 bp	1.0	87.8
trnQ(uug)-trnR(acg)_p3	AAAGTCCACAGCCTTACCAC	GGGGTTCGAATCCCTTCTTG	50.0	55.0	57.7	58.2	124-136 bp	1.0	87.8
trnQ(uug)-trnR(acg)_p4	GTGGCAGGATTCGAACCTAC	GGGGTTCGAATCCCTTCTTG	55.0	55.0	58.3	58.2	157-169 bp	1.0	87.2
trnQ(uug)-trnR(acg)_p5	GTCCACAGCCTTACCACTTG	GGGGTTCGAATCCCTTCTTG	55.0	55.0	58.5	58.2	121-133 bp	1.0	86.2