Hotspot

ndhE

SSC · 118214-118519 · 306 bp

Pi 0.00392

MarkerSeek score 28.70

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 3
pi: 0.00392
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TACATCCGTCCCAGGGTATC	GCGTTAATAGGTGCGATTGC	55.0	50.0	58.0	58.0	933-953 bp	1.0	84.2
ndhE_p2	TACATCCGTCCCAGGGTATC	TTTCCTCTTTGGACCGTTGG	55.0	50.0	58.0	58.0	1161-1181 bp	1.0	83.6
ndhE_p3	AAATTACGGAGCCTGCGAAT	GCGTTAATAGGTGCGATTGC	45.0	50.0	58.0	58.0	1255-1275 bp	1.0	83.1
ndhE_p4	TACATCCGTCCCAGGGTATC	TCTTTCTGGGATCGGGTCTT	55.0	50.0	58.0	58.0	1391-1411 bp	1.0	82.9
ndhE_p5	AAATTACGGAGCCTGCGAAT	TTTCCTCTTTGGACCGTTGG	45.0	50.0	58.0	58.0	1483-1503 bp	1.0	82.7